Monday, August 8, 2011

Molecular work- Diluting primers & PCR amplification

primers, diluted ones

samples on PCR


preparation of samples for PCR amplification

green mastermix, added into the samples to run for PCR

adding primers into tubes

nuclease free water, will be added into the tubes

workplace, full with tips, micropippettes, tubes

tubes labelled

primers before dilution takes place

Arrived Puncak Alam at around 9.30am, went straight to Dr Kala's Lab level 7.
Task done today:

  1. Preparation of primers
  2. Diluting primers, origin form of primers-powder form from Next Gene. 8 pairs of primers altogether.
  3. preparation for PCR..quite complicated coz i have two different samples, from reactor 1 and reactor 2.
I keep on making mistake as i always forgot whether i have added primers, or DNA or nuclease free water...and its so tiring...damn tired..

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