Academic writing, Puteri Resort.

Academic writing workshop takes place at the Puteri Resort, Ayer Keroh, Melaka...

The objective of the workshop was to produce about 12 papers for the VCSP grant.

Ive prepared one, so further discussion and correction were made during the workshop..

Its our 1st trip to some other places besides uitm..! weee, get excited coz at least its outside uitm..

I sleep with Kak satira, went there with Prof, cm, tay, liew, kak salina, kak amani...

n we also make a surprise birthday for Prof (not even a surprise, hahah just 2 choc twiggies with candles) thats the only thing we have..hahah funny!!!

overall, its a great one!

Distributing kad raya

2 days in library Shah Alam

We went to Library shah alam for two days, sat and sunday..

Its a new library, just moved from Sect 14 to Sect 13 Shah Alam, located inside the Golf Club.

Moving my instrument, IC by Metrohm people

Prepare a new set of diluted DNA with 8 pairs of primers

Running PCR product on Gel electrophoresis

gel ready to be viewed with the bio-rad system

done.after running for 40mins, 80 volts

running

well filled with all samples

well been filled up with samples

done for 1 well

placing the mixture of ladder and dye into well

mixing the dye and ladder

the compartment should be filled with TAE buffer so that at least the gel is not so dry

Preparation of Agarose gel

what u need to do???

well, u need to have the set up 1st, then only u can prepare the gel..n dont forget the chemicals

chemicals required:

1. agarose
2. ethidium bromide/EtBr/Bromoethane
3. TAE buffer (contains tris base, EDTA and acetic acid)

all chemicals are mixed well according to the given method..

Molecular work- Diluting primers & PCR amplification

primers, diluted ones

samples on PCR

preparation of samples for PCR amplification

green mastermix, added into the samples to run for PCR

adding primers into tubes

nuclease free water, will be added into the tubes

workplace, full with tips, micropippettes, tubes

tubes labelled

primers before dilution takes place

Arrived Puncak Alam at around 9.30am, went straight to Dr Kala's Lab level 7.

Task done today:

1. Preparation of primers
2. Diluting primers, origin form of primers-powder form from Next Gene. 8 pairs of primers altogether.
3. preparation for PCR..quite complicated coz i have two different samples, from reactor 1 and reactor 2.

I keep on making mistake as i always forgot whether i have added primers, or DNA or nuclease free water...and its so tiring...damn tired..

mmol

Excitement of doing research

molecular techniques

Scrapping biofilm, ayuh!

Talk on Effective Microorganisms

organized by Faculty of Agriculture, on friday, 3-5pm.

by Mr Sawada, expertise on EM.

Moving, again n again

Moving again, n again..non-stop since last year! Im a nomad! together with my reactors! sad!